DNA-Seq with Custom Hybrid Capture Enrichment

DNA-Seq with Custom Hybrid Capture Enrichment

Let Syntezza prepare for you a DNA-Seq library enriched using a custom designed Hybrid Capture panel. Once the library is prepared, we can arrange for sequencing and bioinformatic analysis of the results.

Custom target enrichment using IDT’s xGen Lockdown Hybrid Capture Pools 

IDT’s Hybrid Capture Pools contain individually synthesized Lockdown Probes pre-formulated to deliver consistent, convenient, best-in-class performance. Develop cost-effective custom panels that are delivered quickly (7–10 business days for orders <2000 probes).

Use IDT’s Design tool to select predesigned or create custom xGen Lockdown Probe Pools and to view pricing.

 

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Description

gDNA extraction

Prior to preparing the DNA-Seq library, we can pick up your  samples and perform extraction of the DNA  from your samples at our facility or you can isolate the gDNA at your lab. DNA extraction service is available from ₪25.00 to ₪40.00 per sample.

If you isolate the gDNA at your lab, we will deliver to your lab the appropriate number of GenTegra tubes required to stabilize your DNA samples for transport to our facilities in Jerusalem and will use GenTegra tubes to safely transport your prepared libraries to the NGS sequencing facility. GenTegra technology enables shipping of DNA samples at ambient temperature without any fear of degradation, sample loss or delay in transport. Shipping with GenTegra tubes is provided at ₪12 per sample.

Preparation of the custom enriched DNA-Seq library

Select the Hybrid Capture Pool for your application, we will procure the reagents from IDT and prepare the enriched DNA-Seq library  at a cost of ₪ 1,600.00, in addition to the cost of the custom panel.

When the library is complete, we will send it for sequencing and offer three options:

NGS Sequencer  Read Size Number of Reads Price 
Nova Seq 6000 1 x 100 bp 650-800 M ₪ 8,880.00
Hi Seq 4000  2 x 150 bp 300-350 M ₪ 6,660.00
Nova Seq 6000 2 x 150 bp 8000 M ₪ 33,300.00

Our NGS specialists will help you select the best options and prepare a custom price quotation. Bioinformatic result analysis services are also available. Contact us today at NGS@syntezza.com or call 02-586-7138 ext. 2.

Additional information

Unit

Extraction, GenTegra Shipping, DNA-Seq Library Prep, Exome Panel Enrichment, Cancer Panel Enrichment, Inherited Disease Panel Enrichment, NGS Sequencing 650 to 800M reads, NGS Sequencing 300 to 350M reads, NGS Sequencing 8000M reads