DNA-Seq with Custom Enrichment

DNA-Seq with Custom Enrichment

Let Syntezza prepare for you a DNA-Seq library enriched using a custom designed panel. Efficient, scalable and cost effective targeted genotyping for SNP Detection, Guide-Seq and Insert location. Once the library is prepared, we can arrange for sequencing and bioinformatic analysis of the results.

We offer three cost-effective custom enrichment options:

  1. IDT’s rhAmpSeq System
  2. IDT’s xGen Lockdown Hybrid Capture System
  3. Tecan Genomics’ Allegro System
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Description

gDNA extraction

Prior to preparing the DNA-Seq library, we can pick up your  samples and perform extraction of the DNA  from your samples at our facility or you can isolate the gDNA at your lab. DNA extraction service is available from ₪25 to ₪40 per sample.

If you isolate the gDNA at your lab, we will deliver to your lab the appropriate number of GenTegra tubes required to stabilize your DNA samples for transport to our facilities in Jerusalem and will use GenTegra tubes to safely transport your prepared libraries to the NGS sequencing facility. GenTegra technology enables shipping of DNA samples at ambient temperature without any fear of degradation, sample loss or delay in transport. The cost of shipping and handling is a minimum ₪ 200 for the order, which includes GenTegra DNA tubes for up to 16 samples.

Preparation of the custom enriched DNA-Seq library

Our NGS specialists will help you select the best option for your project, provide a price quotation, procure the reagent set and prepare the enriched library.

IDT’s rhAmp is a cost-effective system for detecting from 5-5,000 SNP targets. The system uses RNA-base–containing blocked primers (rhAmp primers) a proprietary technology that harnesses the intrinsic properties of the RNase H2 enzyme to recognize and cleave DNA:RNA duplexes. Once the panel is procured, we will complete the library, including incorporation of the enrichment panel, for ₪ 150 per sample.

IDT’s Hybrid Capture Pools contain individually synthesized Lockdown Probes formulated to deliver consistent, convenient, best-in-class performance. Develop custom panels that are delivered quickly (7–10 business days for orders <2000 probes). In addition to custom xGen Lockdown Probe Pools, IDT offers a selection of predesigned Lockdown Probe Pools. Once the pool is procured, we will prepare the DNA-Seq library for ₪440 per sample, followed by incorporation of your panel and performance of the complete hybrid capture procedure for ₪ 800 per pool (each pool includes up to 12 samples).

Tecan Genomics’ Allegro Targeted Genotyping System utilizes single primer enrichment technology (SPET) to create a custom panel for specific detection of 500 to > 100,000 SNPs of interest, from low input of 10ng – 100ng of DNA. Highly flexible design process enables easy addition of new content  and scalable multiplexing of thousands of samples per sequencing run. Once the panel is procured, we will complete the library, including incorporation of the enrichment panel, for ₪ 75 per sample.

Custom Allegro panels are available at the following prices:

~ Number of Targets

Number of Reactions

Price per reaction
 < 5,000 96 84 ₪
 < 5,000 192 74 ₪
25,000 – 50,000 96 102 ₪
 25,000 – 50,000 192 92 ₪
 50,000 – 100,000’s 96 Inquire
 50,000 – 100,000’s 192 Inquire
 25,000 – 100,000’s 384 Inquire
 25,000 – 100,000’s 768 Inquire

Sequencing

When the library is complete, we will send it for sequencing. Note, Allegro enriched libraries utilize custom primers in the sequencing and require a dedicated sequencing run.

Pricing for the three most common sequencing options are as follows:

NGS Sequencer  Read Size Number of Reads Price 
Nova Seq 2 X 150 bp 10 M ₪ 213
Hi Seq
2 x 150 bp 350 M ₪ 6,475
Nova Seq
1 x 100 bp 800 M ₪ 22,200

Bioinformatic result analysis services are also available. Contact us today at NGS@syntezza.com or call 02-586-7138 ext. 4.