Guide-Seq

Guide-Seq

12.00750.00

Guide-Seq is a popular method for delineating off-target cleavage activity introduced by CRISPR. First described in Nature Biotechnology, 33:2, 2015, Guide-Seq remains the most rigorous framework to date for genome-wide identification of off-target effects from CRISPR RNA-guided nucleases.

Guide-Seq can also be used to identify the location of DNA inserted by transposomes or other insertion methods.

Click here to see the Guide-Seq protocol.

 

SKU: N/A Category:
Image Variation SKU Price Quantity Add To Cart
Extraction 1 24 Samples G-S Extract 1-24 40.00
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Extraction 25 48 Samples G-S Extract 25-48 35.00
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Extraction 49 96 Samples G-S Extract 49-96 30.00
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Extraction > 96 Samples G-S Extract 96+ 25.00
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Library Prep G-S Prep 750.00
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NGS Sequencing G-S Sequencing 666.00
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Bioinformatics G-S Analysis 266.50
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Ship GenTegra G-S Ship 12.00
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Description

gDNA extraction and transport to Syntezza

Prior to preparing the Guide-Seq library, we can pick up your  samples and perform the DNA  extraction at our facility or you can isolate the gDNA at your lab. DNA extraction service is available from ₪25.00 to ₪40.00 per sample.

If you isolate the gDNA at your lab, we will deliver to your lab the appropriate number of GenTegra tubes required to stabilize your DNA samples for transport to our facilities in Jerusalem and will use GenTegra tubes to safely transport your prepared libraries to the NGS sequencing facility. GenTegra technology enables shipping of DNA samples at ambient temperature without any fear of degradation, sample loss or delay in transport. Shipping with GenTegra tubes is provided at ₪12 per sample.

Library preparation

We maintain all of the reagents required for Guide-Seq library preparation at our facility and will use them and prepare your Guide-Seq library for ₪750 per sample.

NGS sequencing and bioinformatics

For Guide-Seq libraries, we offer NGS sequencing of 1 million reads for ₪ 666 per sample.

Bioinformatic analysis of Guide-Seq sequencing results is provide for ₪ 266.50 per sample.

To obtain a price quotation, simply click on the Price Quote Tab, enter the  quantity of samples, click Add to Cart for each service you wish for us to perform and then click View Cart.

Additional information

Unit

Extraction 1-24 samples, Extraction 25-48 samples, Extraction 49-96 samples, Extraction > 96 samples, Library Prep, NGS Sequencing, Ship GenTegra, Bioinformatics

Price Quote