Microbiome

Microbiome

12.0090.00

Microbiome refers to NGS amplicon sequencing of PCR products reflecting the 16S gene, 18S gene and/or ITS gene of genomic DNA. Starting with extraction of the gDNA, we can prepare the microbiome amplicon library using validated stock primers and arrange for NGS sequencing and bioinformatic analysis of the results.

SKU: N/A Category:
Image Variation SKU Price Quantity Add To Cart
Extraction 1 24 Samples - Microbiome Extract 1-24 40.00
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Extraction 25 48 Samples - Microbiome Extract 25-48 35.00
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Extraction 49 96 Samples - Microbiome Extract 49-96 30.00
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Extraction > 96 Samples - Microbiome Extract 96+ 25.00
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Library Prep 1 24 Samples - Microbiome Prep 1-24 86.00
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Library Prep 25 48 Samples - Microbiome Prep 25-48 80.00
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Library Prep 49 96 Samples - Microbiome Prep 49-96 75.00
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Library Prep >96 Samples - Microbiome Prep 96+ 70.00
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Sequencing 2x250bp 100K Reads - Microbiome Seq 2x250 100K 90.00
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Ship GenTegra - Microbiome ship 12.00
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Description

gDNA extraction

Prior to preparing the microbiome amplicon library, we can pick up your  samples and perform the DNA  extraction at our facility or you can isolate the gDNA at your lab. DNA extraction service is available from ₪25.00 to ₪40.00 per sample.

If you isolate the gDNA at your lab, we will deliver to your lab the appropriate number of GenTegra tubes required to stabilize your DNA samples for transport to our facilities in Jerusalem and will use GenTegra tubes to safely transport your prepared libraries to the NGS sequencing facility. GenTegra technology enables shipping of DNA samples at ambient temperature without any fear of degradation, sample loss or delay in transport. Shipping with GenTegra tubes is provided at ₪12 per sample.

Preparation of the microbiome amplicon library

We offer microbiome amplicon library preparation for ₪70.00 to ₪86.00 per sample and include the stock primers at no additional charge.

For 16s bacterial rRNA amplicons, we recommend use of the following stock primer set:

16s Forward: 976F – CNACGCGAAGAACCTTANC         16s Reverse: 1046R – CGACAGCCATGCANCACCT

For 18s, ITS or alternative 16s stock primer set options – inquire.

NGS sequencing

For microbiome amplicon libraries designed using the recommended 16s stock primer set, we offer sequencing of 2 x 250 pb with 100,000 reads, using the Illumina MiSeq instrument for ₪ 91.00.

For microbiome amplicon libraries designed using 18s, ITS or alternative 16s primer sets, the optimal NGS sequencing option will vary dependent upon amplicon length. Contact our NGS specialists for guidance.

Bioinformatic result analysis services are also available. Contact our NGS specialists for bioinformatics pricing. Contact us today at NGS@syntezza.com or call 02-586-7138 ext. 2.

Additional information

Unit

Extraction 1-24 samples, Extraction 25-48 samples, Extraction 49-96 samples, Extraction > 96 samples, Library Prep 1-24 samples, Library Prep 25-48 samples, Library Prep 49-96 samples, Library Prep >96 samples, Sequencing 2x150bp 100K reads, Sequencing 2x150bp 25M reads, Sequencing 2x250bp 100K reads, Sequencing 2x300bp 100K reads, Ship GenTegra

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