Whole Genome or Validated Panel Enrichment
We provide standard whole genome DNA-Seq library preparation and also offer DNA-Seq library preparation enriched using predesigned panels. We will send your sample for sequencing and offer bioinformatic analysis of the results.
Prior to preparing the DNA-Seq library, we can pick up your samples and perform the DNA extraction at our facility or you can isolate the gDNA at your lab. DNA extraction service is available from ₪25.00 to ₪40.00 per sample.
If you isolate the gDNA at your lab, we will deliver to your lab the appropriate number of GenTegra tubes required to stabilize your DNA samples for transport to our facilities in Jerusalem and will use GenTegra tubes to safely transport your prepared libraries to the NGS sequencing facility. GenTegra technology enables shipping of DNA samples at ambient temperature without any fear of degradation, sample loss or delay in transport. The cost of shipping and handling is a minimum ₪ 200 for the order, which includes GenTegra DNA tubes for up to 16 samples.
DNA-Seq library prep with fragmentation, with or without the incorporation of an enrichment panel: ₪ 440.00 per sample.
IDT’s xGen Exome Research Panel v2
IDT’s exome panel incorporates hybrid capture technology using 415,115 pooled individually synthesized Lockdown Probes. IDT’s exome panel provides the most consistent performance and complete coverage of any exome panel on the market.
Syntezza maintains IDT’s xGen Exome Research Panel in stock. Following preparation of your DNA-Seq library with fragmentation (₪440.00 per sample), we will perform the complete hybrid capture procedure (including provision of the panel, the other hybrid capture reagents and the laboratory service) for a fixed price of ₪ 1,600 per reaction (each reaction includes up to 12 samples).
xGen Predesigned Gene Capture Pools
xGen Predesigned Gene Capture Pools contain individually synthesized Lockdown Probes pre-formulated into individual gene pools for easy selection and setup of custom target panels. Generate deep and uniform coverage data through superior hybridization capture performance, you can choose only the genes you want and obtain full exon coverage for each gene in your pool. Select the Predesigned Gene Capture Pool that best meets your research goals and we order it from IDT at the following price:
|Panel Size||16 rxn||96 rxn|
|10–29 genes||₪ 12,085.00 NIS||₪ 26,185.00 NIS|
|30–49 genes||₪ 14,100.00 NIS||₪ 40,280.00 NIS|
|50–99 genes||₪ 14,105.00 NIS||₪ 56,395.00 NIS|
|100–199 genes||₪ 17,790.00 NIS||₪ 72,510.00 NIS|
|200–399 genes||₪ 21,820.00 NIS||₪ 100,705.00 NIS|
|400–599 genes||₪ 26,855.00 NIS||₪ 138,975.00 NIS|
|600–799 genes||₪ 31,890.00 NIS||₪ 167,170.00 NIS|
|800–1000 genes||₪ 36,925.00 NIS||₪ 191,340.00 NIS|
Once your Gene Capture Pool is procured, we will prepare your DNA-Seq library with fragmentation (₪ 440.00 per sample). We will then perform the complete hybrid capture procedure (including incorporation of your purchased panel, plus provision of the other hybrid capture reagents and the laboratory service) for ₪ 800 per reaction (each reaction includes up to 12 samples).
When your enriched library is complete, we will send it for sequencing. Pricing for the two most common sequencing options are as follows:
|NGS Sequencer||Read Size||Number of Reads||Price|
|Nova Seq||2 x 150 bp||10 M||₪ 213|
||2 x 150 bp||350 M||₪ 6,475|
||1 x 100 bp||800 M||₪ 22,200
Our NGS specialists will help you select the best options and prepare a custom price quotation. Bioinformatic result analysis services are also available. Contact us today at NGS@syntezza.com or call 02-586-7138 ext. 2.