RNA-Seq & mRNA-Seq

RNA-Seq & mRNA-Seq

14.006,475.00

RNA-Seq provides fresh biological insight into the transcriptome, the entire set of RNA transcripts in a given cell for a specific developmental stage or physiological condition. It can be used to determine the structure of genes, their splicing patterns and other post transcriptional modifications, to detect rare and novel transcripts, and to quantify the changing expression levels of each transcript.

RNA-Seq can be also be used to assess mRNA expression in various disease states and changes in mRNA expression as a result of CRISPR or other gene editing interventions.

 

SKU: N/A Category:
Image Variation SKU Price Quantity Add To Cart
Extraction 1 24 Samples RNA-S Extract 1-24 80.00
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Extraction 25 48 Samples RNA-S Extract 25-48 70.00
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Extraction 49 96 Samples RNA-S Extract 49-96 60.00
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Extraction > 96 Samples RNA-S Extract >96 50.00
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RNA Seq Prep Poly A Capture RNA-S Prep polyA Capture 440.00
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RNA Seq Prep Prokaryot RNA-S Prep Prokaryot 600.00
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RNA Seq Prep SoLo RNA-S Prep SoLo 700.00
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RNA Seq Prep Trio RNA-S Prep Trio 950.00
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Sequencing 2x150 350M Reads RNA-S Sequencing 2x150 350M 6,475.00
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Sequencing 2x150 10M Reads RNA-S Sequencing 2x150 10M 213.00
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Sequencing 1x50 20M Reads RNA-S Sequencing 1x50 20M 710.00
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GenTegra Shipping RNA-S Ship 14.00
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Description

RNA extraction and shipping 

Prior to preparing the RNA-Seq library, we can pick up your  samples and perform the RNA extraction at our facility or you can isolate the RNA at your lab. RNA extraction service is available from ₪50 to ₪80 per sample.

GenTegra technology enables shipping of RNA samples at ambient temperature without any fear of degradation, sample loss or delay in transport.

If you isolate the RNA at your lab, we will deliver to your lab the appropriate number of GenTegra tubes with instructions for stabilizing the extracted RNA samples. When your RNA samples are ready, we will return to your lab and transport the RNA to our facility for library preparation. We will also use GenTegra tubes to safely transport your prepared libraries to the NGS sequencing facility. The cost of shipping and handling is a minimum ₪ 200 for the order, which includes GenTegra RNA tubes for up to 14 samples.

Library preparation

We offer five RNA library prep options:

  • RNA Seq with poly A capture Transcriptome for ₪ 440 per sample.
    • This process is appropriate for assessment of mRNA from eukaryotic organisms
  • Prokaryot RNA Seq with rRNA Depletion, for ₪ 600 per sample. 
    • Separate versions are available for assessment of RNA from Human, Mouse, Drosophilia and general prokaryotic organisms
  • SoLo RNA Seq with rRNA Depletion, for ₪ 700 per sample.
    • Solution for ultra-low input or single cell (10 pg to 10 ng of total RNA or cell lysates from 1-500 cells).
  • Trio RNA-Seq  with rRNA Depletion, for ₪ 950 per sample.
    • Solution for liquid biopsy, FFPE and other challenging samples with low input (500 pg – 50 ng of total RNA).
  • RNA Seq with custom select RNA Depletion – inquire for pricing.

NGS Sequencing

When the library is complete, we will send it for sequencing and offer three options:

NGS Sequencer  Read Size Number of Reads Price
Hi SeqX 2 x 150 bp 350 M  ₪ 6475
Nova Seq 1 x 50 bp 20 M  ₪ 710
Nova Seq
2 x 150 bp 10 M ₪ 213

Bioinformatic result analysis services are also available. Contact our NGS specialists for bioinformatics pricing. Contact us today at NGS@syntezza.com or call 02-586-7138.

Additional information

Unit

Extraction 1-24 samples, Extraction 25-48 samples, Extraction 49-96 samples, Extraction > 96 samples, RNA-Seq Prep Poly A Capture, RNA-Seq Prep Prokaryot, RNA-Seq Prep SoLo, RNA-Seq Prep Trio, Sequencing 2×150 350M reads, Sequencing 1×50 20M reads, Sequencing 2×150 10M reads, GenTegra Shipping

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