Ultra Small DNA or RNA Samples
Challenging samples with low input of RNA or DNA require unique NGS library preparation. These include cell-free DNA, purified DNA, RNA or cDNA generated from cell lines, fresh and FFPE tissue, liquid biopsy and fragmented samples.
We have the specialized library prep kits and the experience required to meet these challenges.
We can prepare an effective NGS library from input levels as low as 500 pg – 50 ng of total RNA or 10 pg of DNA.
|Image||Variation||SKU||Price||Quantity||Add To Cart|
|Library Prep -||Ultra low DNA per sample||₪950.00||
||Add to cart|
|Library Prep -||Ultra low RNA per sample||₪950.00||
||Add to cart|
DNA or RNA extraction and shipping
The first step in this process is efficient isolation of the DNA or RNA. We can pick up your samples and perform the DNA or RNA extraction at our facility or you can isolate the DNA or RNA at your lab. DNA and RNA extraction services are available from ₪25.00 to ₪80.00 per sample.
GenTegra technology enables shipping of DNA or RNA samples at ambient temperature without any fear of degradation, sample loss or delay in transport.
If you isolate the DNA or RNA at your lab, we will deliver to your lab the appropriate number of GenTegra DNA or RNA tubes with instructions for stabilizing the extracted DNA or RNA. When you are ready, we will return to your lab and transport the DNA or RNA to our facility for library preparation. We will also use GenTegra tubes to safely transport your prepared libraries to the NGS sequencing facility. Shipping with GenTegra DNA is provided at ₪12 per sample, while GenTegra RNA is provided at ₪13 per sample.
Ultra low sample RNA-Seq library preparation is performed using the NuGEN Trio Low input RNA-Seq that combines three powerful technologies:
- Single Primer Isothermal Amplification (SPIA) enabling access to limited and degraded samples.
- Enzymatic fragmentation and DimerFree library construction allowing efficient and robust library preparation.
- AnyDeplete customizable transcript depletion after library construction maximizing informative sequencing reads from whole transcriptome data.
This process includes Unique Dual Indexes conveniently configured in a pre-plated format as 96 unique barcode pairs. This unique workflow provides whole transcriptome libraries with no adaptor dimers and integrated transcript depletion enabling increased detection and more uniform coverage of transcripts.
Trio low input RNA-Seq is offered with AnyDeplete probes targeting Human rRNA. AnyDeplete probe sets can be customized to any transcript from any organism.
Ultra low sample DNA-Seq library preparation is performed using NuGEN’s Ovation® Ultralow V2 DNA-Seq library preparation kit containing proprietary DimerFree™ technology that allows for efficient library preparation with virtually no adaptor dimers. This process ican be performed using a dedicated single index or Unique Dual Indexes adaptors for each reaction and provides high-fidelity amplification across a broad range of GC content.
When the library is complete, we will send it for sequencing and facilitate bioinformatic results analysis.
Our NGS specialists will help you select the best options and prepare a custom price quotation. Contact us today at NGS@syntezza.com or call 02-586-7138 ext. 2.
Library Prep, NGS Sequencing 130M reads, NGS Sequencing 130M reads, NGS Sequencing 400M reads, NGS Sequencing 400M reads, Bioinformatics