Small RNA species generally include the most common and well-studied microRNA (miRNA), small interfering RNA (siRNA), and piwi-interacting RNA (piRNA), as well as small nucleolar RNA (snoRNA) and small nuclear RNA (snRNA). Small RNA is a type of lowly abundant, short in length (<200 nt), non-protein-coding RNA that lacks polyadenylation. Small RNA populations can vary … Read more
Recommended for samples from human tissue, we offer DNA-Seq library preparation enriched using IDT’s xGen hybridization capture panels (Hyb Panels) targeting your genes of interest. Both predesigned panels and custom panels are available. Hyb Panels are composed of probes having a 5’ biotin group for capture with streptavidin-coated beads. The probes are individually synthesized and … Read more
GBS is the cost-effective method of choice for genome wide SNP discovery without prior knowledge of the genome sequence. GBS enables simultaneous genotyping in multiple DNA samples. It combines restriction enzyme-mediated complexity reduction with the high-throughput sequencing capacity of Illumina platforms to score random markers across an entire genome. GBS enables finding thousands of markers … Read more
Sample extraction, library preparation and whole genome sequencing are available for every type of DNA sample, including samples from plant, animal, human, bacteria or virus. In additional, sequencing of microorganisms from environmental samples such as soil or water, termed Shotgun Metagenomic, can be performed. We utilize different library preparation kits depending upon sample type, quantity … Read more
We have found the Allegro system from Tecan Genomics to be the most efficient and cost-effective option for creating custom panels for specific detection of 500 to over 100,000 SNPs in areas of interest. This system utilizes single primer enrichment technology (SPET) and can be applied to as little initial input as 200ng of DNA. … Read more
DNA methylation is the most widely studied epigenetic modification. DNA methylation occurs primarily on cytosine bases in the CpG dinucleotide motif in most mammalian cells, and across all cytosine contexts in plant genomes. DNA methylation is often associated with the regulation of gene expression through its presence or absence in CpG islands among gene promoter … Read more
IDT’s exome panel provides the most consistent performance and complete coverage of any exome panel on the market. It consists of 415,115 individually synthesized, 5’ biotinylated, oligonucleotide probes. Each probe was quality tested and combined so that the panel contains the correct concentration. Syntezza maintains IDT’s exome panel in stock. Following preparation of your DNA-Seq … Read more
RNA-Seq provides fresh biological insight into the transcriptome, the entire set of RNA transcripts in a given cell for a specific developmental stage or physiological condition. It can be used to determine the structure of genes, their splicing patterns and other post transcriptional modifications, to detect rare and novel transcripts, and to quantify the changing … Read more
Amplicon sequencing is a method for preparing focused NGS libraries from gDNA using PCR reactions. Single amplicons A first PCR process utilizes custom PCR primers to generate the amplicon that will be the focus of the sequencing designed. The primers amplify your specific region of interest plus an additional 20 bp region upstream of each … Read more