Macrogen’s Sequencing Services

  • Sanger Sequencing for Plasmid segments or PCR products
  • Oxford Nanopore Sequencing for Whole Plasmids
  • Plasmid pickup every Sunday and Monday
  • Purification prior to Sanger sequencing
  • Individual tubes or 96-well plates
  • Sanger pickup daily Sunday-Thursday (from most institutions)
  • Sanger Read length up to 1,050 bases
  • Free re-sequencing of tubes (with Macrogen’s approval)
  • Submit unique primers, or use Macrogen’s in-stock universal primers

 

How to Order:

Step 1: Go to the Macrogen website, and create a new Macrogen Customer Account. Click here to view a Guide for Creating a Macrogen Customer Account.

*Please note that to order Macrogen Sequencing Services, you need to create a customer account on the Macrogen website, even if you have have a Syntezza customer account. Sanger Sequencing orders must be submitted on the Macrogen website using a Macrogen Customer Account.

Step 2:

When your samples are ready for sequencing, login to your customer account on the Macrogen website and place your order. All orders should be selected under the “Ordering” menu. Make sure that you are not using the “Quoting” menu instead.

User guides for placing online orders and preparing the samples are available on the Macrogen website.

*Please note that all orders from Israel are automatically scheduled for express turnaround service. Syntezza will invoice you for your orders at the end of each month.

Step 3: A Syntezza driver will pick-up your samples.

When you submit an order on the Macrogen website, Syntezza is notified. You do not need to contact us for pick-up.

Save more with barcode services:

Save time and money by using our advanced Eco-Seq or EZ-Seq barcode labels to place your order. Purchase packs of 50 barcodes and use them to send samples at a reduced cost per sample. To learn more, including guides for ordering, visit Barcode-Services.

Once you have purchased Eco or EZ labels, instead of placing  individual orders on the Macrogen website, simply let our offices know each time you have samples ready for pick-up. Contact us by email at sequencing@syntezza.com or call 02-586-7138.

Prices:

Download complete Price List

Whole Plasmid Sequencing with Oxford Nanopore

  • 120 NIS per sequence

PCR product and Plasmid Sanger sequencing (Tubes & Plates)

Tubes (TAT 48 hours)

  • 29 NIS/sample

Plates (TAT 3-5 work days)

  • 1 primer/1 plate: 2,000 NIS
  • 1 primer/ half-plate: 1,200 NIS
  • Multiple* primers/1 plate: 2,200 NIS

*Maximum of 6 primers

Sanger Sequencing with Labels (TAT 48 hours)

  • Eco-seq labels: Minimum of 50 labels for 1,200 NIS (or 24 NIS/reaction)
  • Eco-seq labels sequencing plus purification: Minimum of 50 labels for 1,600 NIS (or 32 NIS/ reaction)
  • Eco-seq 96-well Plate: Minimum of 2 plate labels for 1,800 NIS/label
  • Eco-seq 96-well Plate sequencing plus purification: Minimum of 2 plate labels for 2,000 NIS/label
  • EZ-seq labels: Minimum of 50 labels for 1,200 NIS (or 24 NIS/reaction)
  • EZ-seq 96-well Plate: Minimum of 2 plate labels for 1,500 NIS/label

*Eco-seq plates have a maximum of 4 primers.

*Please note that purification adds 24 hours to the TAT.

Extra Services (TAT 3-5 work days)

  • Difficult Sequencing: 50 NIS/sample
  • Primer Synthesis: 6 NIS per base
  • 16s rRNA, ITS region or 18S rRNA: 240 per reaction
  • PCR product Purification: 12 NIS/sample
  • PCR Gel Extraction: 40 NIS/sample
  • 96-well Plate Purification: 650 NIS/plate

*Please note that a minimum of 20 uL is required for purification services. Macrogen performs filter purification, but they can perform purification by Exo-sap (chemical purification), upon the customer’s request.

 

FAQs:

  1. The concentration of my sample is not high enough, what should I do?

If your concentration is somewhat close to what is required, then you can send a larger volume than what is recommended. However, please write on your order form that you included a slightly lower concentration of sample.

Also, please note that Macrogen only performs re-sequencing if they feel that the low quality of the results was due to their own technical errors. Re-sequencing is not available for samples in Plates or Labels.

  1. I want my sample to be sequenced by multiple primers. Do I still need to send in only 20 microliters?

Macrogen uses about 5 microliters of sample DNA per reaction. However, it is always better to send more than required. For example, if your order requires 25 microliters of reaction, try to send at least 30 microliters, if not more.

  1. Are there any limits to using multiple primers for a 96-well plate?

The 1 primer/1 plate service requires that all samples in the 96-well plate are to be sequenced with the same primer.  Multiple primers/1 plate service allows for up to 6 primers per plate. However, this service stipulates that individual wells be sequenced with only 1 primer. If the DNA in an individual well is to be sequenced with multiple primers, each sequencing reaction incurs a charge for a single plate sequencing service.

For technical assistance, contact biosupport@syntezza.com or call 02-586-7138 ext. 4.