Macrogen’s Express Sanger and Oxford Nanopore Sequencing Services

  • Sanger Sequencing for Plasmid segments or PCR products
  • Oxford Nanopore Sequencing for Whole Plasmids
  • Plasmid pickup every Sunday and Monday
  • Purification prior to Sanger sequencing
  • Individual tubes or 96-well plates
  • Sanger pickup daily Sunday-Thursday (from most institutions)
  • Sanger Read length up to 1,050 bases
  • Free re-sequencing of tubes (with Macrogen’s approval)
  • Submit unique primers, or use Macrogen’s in-stock universal primers

 

Note: Due to the war in Gaza, many international airlines and cargo planes are not currently flying to Israel. This has caused delays in transport of samples to Macrogen and in our receipt of custom products from our international suppliers. We appreciate your patience during these challenging days.    

How to Order:

Step 1: Go to the Macrogen website, and create a new Macrogen Customer Account. Click here to view a Guide for Creating a Macrogen Customer Account.

*Please note that to order Macrogen Sequencing Services, you need to create a customer account on the Macrogen website, even if you have have a Syntezza customer account. Sanger Sequencing orders must be submitted on the Macrogen website using a Macrogen Customer Account.

Step 2: When your samples are ready for sequencing, login to your customer account on the Macrogen website and place your order. Here is a Guide for Entering Sanger Sequencing Orders on the Macrogen website.

Note: All services for customers in Israel should be ordered with Express turnaround

See here for the requirements of sample preparation, also shown briefly below.

To order 1 primer/1 plate sequencing, there is a template directly on the Macrogen Website.

Click here to download an Excel template to order multiple primers/1 plate sequencing.

*Please note that Syntezza will invoice you for your orders at the end of each month.

Step 3: A Syntezza driver will pick-up your samples.

When you submit an order on the Macrogen website, Syntezza is notified. You do not need to contact us for pick-up.

Save time and money by using our advanced Eco-Seq or EZ-Seq  labels to place your order. Once you have purchased Eco or EZ labels, instead of placing  individual orders on the Macrogen website, simply let our offices know each time you have samples ready for pick-up. Contact us by email at sequencing@syntezza.com or call 02-586-7138 ext. 3 (Rivka).

Prices:

Download complete Price List

Whole Plasmid Sequencing with Oxford Nanopore

  • 120 NIS per sequence

PCR product and Plasmid Sanger sequencing (Tubes & Plates)

Tubes (TAT 48 hours)

  • 34 NIS/sample

Plates (TAT 3-5 work days)

  • 1 primer/1 plate: 2,000 NIS
  • Multiple* primers/1 plate: 2,500 NIS

*Maximum of 6 primers

Sanger Sequencing with Labels (TAT 48 hours)

  • Eco-seq labels: Minimum of 50 labels for 1,200 NIS (or 24 NIS/reaction)
  • Eco-seq labels sequencing plus purification: Minimum of 50 labels for 1,400 NIS (or 28 NIS/ reaction)
  • Eco-seq 96-well Plate: Minimum of 2 plate labels for 1,600 NIS/label
  • Eco-seq 96-well Plate sequencing plus purification: Minimum of 2 plate labels for 1,800 NIS/label
  • EZ-seq labels: Minimum of 50 labels for 1,200 NIS (or 24 NIS/reaction)
  • EZ-seq 96-well Plate: Minimum of 2 plate labels for 1,500 NIS/label

*Eco-seq plates have a maximum of 4 primers.

*Please note that purification adds 24 hours to the TAT.

Extra Services (TAT 3-5 work days)

  • Difficult Sequencing: 42 NIS/sample
  • Primer Synthesis: 4 NIS per base
  • 16s rRNA, ITS region or 18S rRNA: 330 per reaction
  • PCR product Purification: 10 NIS/sample
  • PCR Gel Extraction: 21 NIS/sample
  • 96-well Plate Purification: 490 NIS/plate
  • Fragment Analysis: 15.2 NIS/reaction or 950 NIS/plate

*Please note that a minimum of 20 uL is required for purification services. Macrogen performs filter purification, but they can perform purification by Exo-sap (chemical purification), upon the customer’s request.

Basic Requirements for Sample Preparation:

Sanger Plasmid:

3-4 KB plasmid: 100-150ng/ul

>4KB: >150ng/ul

>10KB:>500ng/ul

Over 15KB, use Oxford Nanopore Sequencing of whole plasmid.

*Minimum volume of 20µl

PCR products:

Purified PCR products

Volume: 5-10µl

Concentration:

– Over 700bp: higher than 50ng/ul

– 300bp~700bp: 25-50ng/ul

– Less than 300bp: 10-15ng/µl

Unpurified PCR products

Volume: at least 20µl

Concentration:

– Over 700bp: higher than 70ng/µl

– 300bp~700bp: 50ng/µl

– Less than 300bp: 20-25ng/µl

Primer concentration: 5-10 pmole/ul, and please always inform Macrogen of the size of the template. *A list of universal primers kept in-house at Macrogen can be found here.

*Following a quick check by Nanodrop, the sample concentration must be confirmed by gel electrophoresis.

Download our complete guide How to Prepare Samples for Macrogen Sanger Sequencing 

FAQs:

  1. The concentration of my sample is not high enough, what should I do?

If your concentration is somewhat close to what is required, then you can send a larger volume than what is recommended. However, please write on your order form that you included a slightly lower concentration of sample.

Also, please note that Macrogen only performs re-sequencing if they feel that the low quality of the results was due to their own technical errors. Please see Macrogen’s troubleshooting guide for a list of sequencing failures that do not quality for free re-sequencing.

  1. I want my sample to be sequenced by multiple primers. Do I still need to send in only 20 microliters?

Macrogen uses about 5 microliters of sample DNA per reaction. However, it is always better to send more than required. For example, if your order requires 25 microliters of reaction, try to send at least 30 microliters, if not more.

  1. Are there any limits to using multiple primers for a 96-well plate?

The 1 primer/1 plate service requires that all samples in the 96-well plate are to be sequenced with the same primer.  Multiple primers/1 plate service allows for up to 6 primers per plate. However, this service stipulates that individual wells be sequenced with only 1 primer. If the DNA in an individual well is to be sequenced with multiple primers, each sequencing reaction incurs a charge for a single plate sequencing service.

For technical assistance, contact biosupport@syntezza.com or call 02-586-7138 ext. 5.