DNA methylation is the most widely studied epigenetic modification. DNA methylation occurs primarily on cytosine bases in the CpG dinucleotide motif in most mammalian cells, and across all cytosine contexts in plant genomes. DNA methylation is often associated with the regulation of gene expression through its presence or absence in CpG islands among gene promoter regions, and these methylation patterns can be used to identify different cell types and disease states.
NGS Syntezza offers 4 types of methylation sequencing utilizing different library prep kits and add on modules.
- WGBS – Whole genome bisulfite sequencing utilizes IDT technology that efficiently captures bisulfite-converted ssDNA molecules into library molecules for epigenetic research studies. The resultant libraries provide uniform methylome and comprehensive genome coverage. An add on module enables constructing an NGS library from bisulfite-converted DNA from single cells.
- WGoxBS – Whole genome oxidative bisulfate sequencing enables discrimination between 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC), enabling absolute quantification of 5mC and 5hmC at single-base resolution.
- RRBS – Reduced Representation Bisulfite Sequencing effectively sequences highly repetitive methylated regions. RRBS is a genome-wide DNA methylation analysis, which only sequences a reduced, representative sample of whole genome, reduces the cost of whole-genome sequencing. Suitable sample input is human genomic DNA from a broad range of cell and tissue types.
- RRoxBS Reduced Representation Oxidative Bisulfite Sequencing enables 5-methylcytosine (5mC) identification and the interrogation of 5-hydroxymethylcytosine (5hmC).