GRS Total RNA Kit – Yeast & Fungus

GRS Total RNA Kit – Yeast & Fungus

2,185.00

Efficient and fast method for the purification of high quality total RNA (including mRNA, tRNA and rRNA) from yeast and a wide variety of fungus species.

SKU: GK17.0100 Category:
GK17.0100

Description

The GRS Total RNA Kit – Yeast & Fungus – provides an efficient and fast method for the purification of high quality total RNA (including mRNA, tRNA and rRNA) from yeast and a wide variety of fungus species. Eluted purified RNA is suitable for RT-PCR, Northern Blotting, mRNA selection, cDNA synthesis, and primer extension.

Principle
The GRS Total RNA Kit – Yeast & Fungus – includes Sorbitol Buffer to reduce sample preparation and thus overall minimizes hands on time. Detergents and chaotropic salts lyse spheroplasts and denature proteins/inactivate RNAse. Optional DNAse treatment can be included in the protocol to remove undesired trace amounts of DNA. The buffer system is optimized to allow selective binding of RNA to the glass fiber matrix of the spin column (1). Contaminants are completely removed using a Wash Buffer (containing ethanol) in a simple centrifugation step. The purified RNA is subsequently eluted with RNAse-Free Water. The entire procedure can be completed within 20 minutes (from spheroplasts) with typical RNA yields of 20- 25 μg (from 5x107cells).

 

Sample : up to 5×107 cells of a large variety of yeast and fungus species
Expected Yield : up to 30 μg total RNA (typically 20-25μg from 5×107 S. cerevisiae)
Format : spin column (certified DNAse/RNAse-free)
Operation Time : 20-30 minutes (from spheroplasts), within 1,5h from cells.
Elution Volume : 50-100μl

Documents

Product Info – GRS total RNA kit – Yeast & Fungus

Price Quote

Reference Description Quantity Price Add to Cart
GK17.0100 GRS Total RNA Kit – Yeast 100 preps/kit ₪100