Xpert cDNA Synthesis Supermix (with gDNA eraser)
The Xpert cDNA Supermix (with gDNA eraser) combines an effective genomic DNA removal reaction mixture with a convenient Reverse Transcriptase mastermix (Xpert RTase Mix).
Presence of trace amounts of contaminating genomic DNA (gDNA) in RNA preparations may lead to significant problems, such as false-positive signals and misjudgment of gene expression levels, hence the effective removal of gDNA prior to cDNA synthesis is essential to ensure reliable results.
The Xpert cDNA Supermix (with gDNA eraser) combines an effective genomic DNA removal reaction mixture with a convenient Reverse Transcriptase mastermix (Xpert RTase Mix). Using gDNA eraser, any residual DNA (ssDNA, dsDNA and plasmid DNA) is eliminated efficiently from the RNA sample in just a few minutes. The reaction is stopped by adding a stop solution, eliminating the need of a heating or organic extraction step that could be prejudicial to the RNA integrity. The gDNA-free RNA solution can then be reverse-transcribed into cDNA directly, using the Xpert RTase Mix.
Xpert RTase Mix is an optimized mastermix containing a balanced concentration of oligo(dT) and random hexamer primers, dNTPs, RNase inhibitor, and Xpert Reverse Transcriptase (RNase H-). In this way, the need for multiple component additions is eliminated and thus the chance of handling errors is reduced, rendering excellent reproducibility and absolute convenience.
In summary, this system provides an efficient and fast method for the synthesis of high-quality, full-length (up to 8-10kb) first strand cDNA with excellent yields from purified poly(A)+ mRNA or total RNA templates.
Please note that, since the mastermix already contains primers, this product cannot be used with gene-specific primers. First strand cDNA can be directly used as template in PCR.
|Reference||Description||Quantity||Price||Add to Cart|
|GK82.0100||Xpert cDNA Synthesis Supermix (with gDNA eraser||100 rxn||₪100|