Acrylamide-Bisacrylamide Solutions

Acrylamide-Bisacrylamide Solutions

250.00273.00

Ready-to-Use Acrylamide-Bisacrylamide solutions are aqueous solutions of ultrapure, 3x re-crystallized acrylamide and molecular biology grade bisacrylamide, prepared with ultrapure water, 0.2 µm filtered, and suitable for electrophoresis of proteins and nucleic acids.

These solutions are available in two concentrations (30% or 40%) and three options of acrylamide to bisacrylamide ratio mixtures (19:1, 29:1 and 37.5:1).

Each solution comes in a 500mL bottle.

 

 

Image Variation SKU Price Quantity Add To Cart
30% (19:1) GB16: 3019 250.00
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30% (29:1) GB16: 3029 250.00
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30% (37.5:1) GB16: 3037 250.00
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40% (19:1) GB16: 4019 273.00
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40% (29:1) GB16: 4029 273.00
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40% (37.5:1) GB16: 4037 273.00
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Description

Principle

The range of  protein sizes to be separated is determined by the pore size of a polyacrylamide gel. Control of the pore size is accomplished by regulating both T and C. The relationship between T and pore size is nearly linear; Gels with a higher percentage of T have smaller pores, and are used to separate smaller proteins. However, the relationship between C and pore size is more complex with a minimum occurring at about 5% bisacrylamide.

A ratio between acrylamide and bisacrylamide of 19:1 (5% C) is suitable for the separation of small peptides, whereas a ratio of 29:1 is commonly used for the separation of “normal sized” proteins. High molecular weight proteins are best separated using a 37,5:1 mix ratio.

The concentration of these products (30% or 40%) are determined by the total (T) weight of both acrylamide and bisacrylamide (T= 30g or T=40g, per 100ml).

  • The mix ratio is 19:1 results in a cross-linking (C) of 5%.
  • The mix ratio of 29:1 results in a cross-linking (C) of 3.3%.
  • The mix ratio is 37.5:1 results in a cross-linking (C) of 2.7%.

Procedure

1. After Western Blotting, image the blot for permanent record, and then rinse the blot with 20ml of water for 5 minutes at room temperature, using an orbital shaker.
2. Decant and incubate the membrane in a container containing 10-20ml of GRS Stripping Buffer (membrane must be completely covered) at room temperature for 5-10 minutes, using an orbital shaker
3. After stripping, wash the membrane extensively (min 3x) with wash buffer (e.g. TBS(T) or PBS(T)).
4. After washing, block the membrane as usual.
5. After blocking the membrane is ready for the next detection with other set of antibodies.

Note: Because Stripping may reduce signal intensity, it is recommended to probe first for the antigen with the lowest level of expression. If more stringent stripping is required, one could add reducing agent (DTT or β-mercaptoethanol) to the stripping buffer and/or heat the blot during the stripping procedure.

For more information, view the Product User Guide for each of the 6 product options (see Documents).

Additional information

Unit

30% (19:1), 30% (29:1), 30% (37.5:1), 40% (19:1), 40% (29:1), 40% (37.5:1)

Documents

Product User Guide– Acrylamide Bisacrylamide 30% (19:1)

Product User Guide – Acrylamide Bisacrylamide 40% (19:1)

Product User Guide – Acrylamide Bisacrylamide 30% (29:1)

Product User Guide – Acrylamide Bisacrylamide 40% (29:1)

Product User Guide – Acrylamide Bisacrylamide 30% (37.5:1)

Product User Guide – Acrylamide Bisacrylamide 40% (37.5:1)

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