ALT-R Cas9 and Cas12 Enzymes

ALT-R Cas9 and Cas12 Enzymes

750.004,000.00

Six recombinant, high-purity endonucleases from IDT for CRISPR genome editing experiments. Nuclear localization signals (NLSs) provide increased genome editing efficiency. Use as part of a ribonucleoprotein (RNP) complex for optimal performance.

  • Alt-R S.p. Cas9 Nuclease V3
  • Alt-R S.p. HiFi Cas9 V3
  • Alt-R A.s. Cas12a (Cpf1) Nuclease V3
  • Alt-R A.s. Cas12a (Cpf1) Ultra
  • Alt-R S.p. Cas9 D10A Nickase V3
  • Alt-R dCas9 protein V3
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Image Variation SKU Price Quantity Add To Cart
ALT-R Cas9 Enzymes Alt R® S.p. Cas9 Nuclease V3 - 100 µg 1081058 750.00
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ALT-R Cas9 Enzymes Alt R® S.p. Cas9 Nuclease V3 - 500 µg 1081059 3,000.00
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ALT-R Cas9 Enzymes Alt R® S.p. HiFi Cas9 Nuclease V3 - 100 µg 1081060 1,000.00
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ALT-R Cas9 Enzymes Alt R® S.p. HiFi Cas9 Nuclease V3 - 500 µg 1081061 4,000.00
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ALT-R Cas9 Enzymes Alt R® S.p. Cas9 D10A Nickase V3 - 100 µg 1081064 750.00
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ALT-R Cas9 Enzymes Alt R® S.p. DCas9 Protein V3 - 100 µg 1081066 750.00
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ALT-R Cas9 Enzymes Alt R® A.s. Cas12a (Cpf1) V3 - 100 µg 1081068 750.00
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ALT-R Cas9 Enzymes Alt R® A.s. Cas12a (Cpf1) V3 - 500 µg 1081069 3,000.00
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ALT-R Cas9 Enzymes Alt R® A.s. Cas12a (Cpf1) Ultra - 100 µg 10001272 1,000.00
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ALT-R Cas9 Enzymes Alt R® A.s. Cas12a (Cpf1) Ultra - 500 µg 10001273 4,000.00
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Description

Cas9 enzymes are high purity recombinant S. pyogenes Cas9 nucleases, purified from an E. coli strain. Cas12a (Cpf1) Nuclease V3 enzyme is highly purified recombinant Acidaminococcus sp. BV3L6 Cas12a. All contain nuclear localization sequence (NLS) and C-terminal 6-His tag and are provided in solution at 10 µg/µL. 100 µg of Cas9 nuclease = 610 pmol.

The S. pyogenes Cas9 enzyme must be combined with a gRNA to produce a functional, target-specific editing complex.

Alt-R S.p. HiFi Cas9 Nuclease V3 offers improved specificity over wild-type Cas9, greatly reducing the risk of off-target cutting events. This Cas9 variant also preserves the high level of editing efficiency expected from a Cas9 nuclease, maintaining 90–100% on-target editing activity at most sites. For applications that are sensitive to off-target events, combining the Alt-R S.p. HiFi Cas9 Nuclease V3 with the optimized Alt-R CRISPR-Cas9 gRNA (crRNA:tracrRNA) is highly recommended.

Alt-R Cas12a (Cpf1) Ultra nuclease has higher on-target potency than the wild-type A.s. Cas12a, and its activity is now as impressive as that of wild-type S.p. Cas9. The Alt-R Cas12a (Cpf1) Ultra also can recognize many TTTT PAM sites in addition to TTTV motifs, increasing target range for genome editing studies. Furthermore, Alt-R Cas12a (Cpf1) Ultra nuclease is active at room temperature, making it a flexible tool for applications requiring delivery at lower temperatures. The wild-type A.s. Cas12a V3 enzyme which recognizes the TTTV PAM site is still available for legacy use.

Alt-R S.p. Cas9 D10A Nickase V3 creates a single-stranded cut in the targeted strand of DNA, and Alt-R S.p. Cas9 nickases allow specific cutting of only one strand at the DNA target site. Cuts to both strands of DNA are accomplished by using either Alt-R S.p. Cas9 D10A Nickase V3 with 2 gRNAs that target two neighboring Cas9 sites, one on either strand of the target region. This functionally increases the length of the recognition sequence from 20 to 40 bases. For more information about using Cas9 nickases, see the application note.

Alt-R S.p. dCas9 Protein V3 has mutations that result in the loss of nuclease activity. This protein can form RNP complexes with Alt-R gRNAs and bind to the target region specified by the gRNA without cutting the DNA.

Additional information

Item

Alt-R® S.p. Cas9 Nuclease V3, Alt-R® S.p. HiFi Cas9 Nuclease V3, Alt-R® S.p. Cas9 D10A Nickase V3, Alt-R® S.p. Cas9 H840A Nickase V3, Alt-R® S.p. dCas9 Protein V3, Alt-R® A.s. Cas12a (Cpf1) V3, Alt-R® A.s. Cas12a (Cpf1) Ultra

Unit

100 µg, 500 µg

Documents

Alt-R CRISPR-Cas9 system—RNP transfections (recommended) (1236 KB)

Alt-R CRISPR-Cas9 system—RNP electroporation, Amaxa Nucleofector system (503 KB)

Alt-R CRISPR-Cas9 system—RNP electroporation, Neon Transfection system (414 KB)

Alt-R CRISPR-Cas9 system—RNP electroporation, Gene Pulser Xcell system (503 KB)

Alt-R CRISPR-Cas9 system—in vitro cleavage of target DNA with RNP complex (420 KB)

Alt-R CRISPR-Cas9 system—plasmid transfections (430 KB)

Alt-R Genome Editing Detection Kit (910 KB)

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