DNase I

DNase I

329.50

DNase I (Deoxyribonuclease I, 29kDa monomer) is an endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, to release di-, tri-, and oligonucleotide products (on average producing tetranucleotides) with 5′-phosphorylated and 3′-hydroxylated ends.

SKU: GE012.0100 Category:
GE012.0100

100mg

Description

DNase I (Deoxyribonuclease I, 29kDa monomer) is an endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, to release di-, tri-, and oligonucleotide products (on average producing tetranucleotides) with 5′-phosphorylated and 3′- hydroxylated ends. DNase I acts on single-stranded DNA, doublestranded DNA, RNA-DNA hybrids, and chromatin.

Applications
Removal of residual genomic DNA from RNA samples, degradation of DNA template in transcription reactions, DNase I footprinting, Nick Translation.

 

Quantity
100 mg lyophilized powder purified from purified from Escherichia coli harbouring the an MBP fusion clone of Bovine Pancreatic DNAse I.

Specifications
Free of RNAses. Specific Activity >2000 Kunitz units per mg.

Storage
Upon arrival, reconstitute DNAse I with 1,0 ml storage buffer at a final concentration of 100mg/ml. Note that DNAse I is sensitive to physical denaturation, therefore, mix genly by inversion and do not vortex. Aliquot and store at -20ºC.

Enzyme activity
For maximal activity, DNAse I requires bivalent cations Ca2+ and Mg2+. pH optimum is in the range of 7.5 to 8.0.

Inhibition
DNAse I is inhibited by metal chelators, transition metals (for example Zn) in mM concentrations, SDS (at concentrations even below 0,1%), reducing agents (DDT, β- mercaptoethanol), and ionic strength above 50-100mM.

Inactivation
DNAse I can be heat-inactivated at temperatures above 65ºC.

Documents

Product Info DNase I

Price Quote

Reference Description Quantity Price Add to Cart
GE012.0100 DNAse I 100mg ₪100