DNase I
DNase I
₪329.50
DNase I (Deoxyribonuclease I, 29kDa monomer) is an endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, to release di-, tri-, and oligonucleotide products (on average producing tetranucleotides) with 5′-phosphorylated and 3′-hydroxylated ends.
100mg
Description
DNase I (Deoxyribonuclease I, 29kDa monomer) is an endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, to release di-, tri-, and oligonucleotide products (on average producing tetranucleotides) with 5′-phosphorylated and 3′- hydroxylated ends. DNase I acts on single-stranded DNA, doublestranded DNA, RNA-DNA hybrids, and chromatin.
Applications
Removal of residual genomic DNA from RNA samples, degradation of DNA template in transcription reactions, DNase I footprinting, Nick Translation.
Quantity
100 mg lyophilized powder purified from purified from Escherichia coli harbouring the an MBP fusion clone of Bovine Pancreatic DNAse I.
Specifications
Free of RNAses. Specific Activity >2000 Kunitz units per mg.
Storage
Upon arrival, reconstitute DNAse I with 1,0 ml storage buffer at a final concentration of 100mg/ml. Note that DNAse I is sensitive to physical denaturation, therefore, mix genly by inversion and do not vortex. Aliquot and store at -20ºC.
Enzyme activity
For maximal activity, DNAse I requires bivalent cations Ca2+ and Mg2+. pH optimum is in the range of 7.5 to 8.0.
Inhibition
DNAse I is inhibited by metal chelators, transition metals (for example Zn) in mM concentrations, SDS (at concentrations even below 0,1%), reducing agents (DDT, β- mercaptoethanol), and ionic strength above 50-100mM.
Inactivation
DNAse I can be heat-inactivated at temperatures above 65ºC.
Documents
Price Quote
| Reference | Description | Quantity | Price | Add to Cart |
|---|---|---|---|---|
| GE012.0100 | DNAse I | 100mg | ₪100 |
