GRS One-Step RT-PCR Kit

GRS One-Step RT-PCR Kit

1,207.00

Using gene-specific primers (GSP), the GRS One-Step RT-PCR Kit allows for first-strand cDNA synthesis and subsequent PCR in a single-tube reaction procedure, decreasing contamination risk and reducing hands-on time considerably.

SKU: GK54.0100 Category:
GK54.0100

100 rxns

Description

Using gene-specific primers (GSP), the GRS One-Step RT-PCR Kit allows for first-strand cDNA synthesis and subsequent PCR  in a single-tube reaction procedure, decreasing contamination risk and reducing hands-on time considerably.

The kit consist of an enzyme mix comprising a genetically modified M-MLV reverse transcriptase with deficient RNAse H activity and improved synthesis efficiency combined with RNAse inhibitor, and a convenient mastermix containing all other required components,  including a high-fidelity DNA polymerase blend, dNTPs and tracking dye allowing for direct loading onto agarose gels for subsequent DNA electrophoresis. PCR products contain 3´ A-overhang and are thus suitable for TA-cloning. The kit allows for the amplification of fragments up to 8kb.

  • Easy Reaction set-up
  • Little hands-on time
  • Single-tube reaction – less contaminations
  • includes High-fidelity DNA polymerase

Kit Contents

Component Volume
GRS RT-PCR Enzyme Mix 40 µl
GRS One-Step RT-PCR Mix 1 ml
RNAse-free water 1 ml

Documents

GRS One-Step RT PCR Kit Info

Price Quote

Reference Description Quantity Price Add to Cart
GK54.0100 GRS One-Step RT-PCR Kit 100 rxns ₪100