Guide-Seq
Guide-Seq
₪12.00 – ₪750.00
Guide-Seq is a popular method for delineating off-target cleavage activity introduced by CRISPR. First described in Nature Biotechnology, 33:2, 2015, Guide-Seq remains the most rigorous framework to date for genome-wide identification of off-target effects from CRISPR RNA-guided nucleases.
Optional iGUIDE process enables a more accurate assessment of the distribution of dsDNA breaks in cells to help clarify the true cleavage signal. iGUIDE relies on the easy-to-use Guide-Seq NGS method utilizing a longer protected oligonucleotide during the CRISPR process and requires a modified NGS results interpretation software.
Guide-Seq can also be used to identify the location of DNA inserted by transposomes or other insertion methods.
Click here to see the Guide-Seq protocol.
| Image | Variation | SKU | Price | Quantity | Add To Cart |
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Extraction 1-24 samples | G-S Extract 1-24 | ₪40.00 |
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Add to cart |
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Extraction 25-48 samples | G-S Extract 25-48 | ₪35.00 |
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Add to cart |
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Extraction 49-96 samples | G-S Extract 49-96 | ₪30.00 |
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Add to cart |
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Extraction > 96 samples | G-S Extract 96+ | ₪25.00 |
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Add to cart |
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Library Prep | G-S Prep | ₪750.00 |
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Add to cart |
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Ship GenTegra | G-S Ship | ₪12.00 |
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Add to cart |
Description
gDNA extraction and transport to Syntezza
Prior to preparing the Guide-Seq library, we can pick up your samples and perform the DNA extraction at our facility or you can isolate the gDNA at your lab. DNA extraction service is available from ₪25.00 to ₪40.00 per sample.
If you isolate the gDNA at your lab, we will deliver to your lab the appropriate number of GenTegra tubes required to stabilize your DNA samples for transport to our facilities in Jerusalem and will use GenTegra tubes to safely transport your prepared libraries to the NGS sequencing facility. GenTegra technology enables shipping of DNA samples at ambient temperature without any fear of degradation, sample loss or delay in transport. The cost of shipping and handling is a minimum ₪ 200 for the order, which includes GenTegra DNA tubes for up to 16 samples.
Library preparation
We maintain all of the reagents required for Guide-Seq library preparation at our facility and will use them and prepare your Guide-Seq library for ₪750 per sample.
NGS sequencing and bioinformatics
Our NGS specialists will help you select the best options and prepare a custom price quotation. Contact us today at NGS@syntezza.com or call 02-586-7138 ext. 4.
Additional information
| Unit | Extraction 1-24 samples, Extraction 25-48 samples, Extraction 49-96 samples, Extraction > 96 samples, Library Prep, Ship GenTegra |
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