IDT xGen Accessories

IDT xGen Accessories

1,500.00104,500.00

Enrichment of a DNA-Seq library with an IDT xGen Lockdown panel or pool requires use of the xGen Hybridization and Wash Kit as well as blocking oligos.

IDT’s xGen Hybridization and Wash Kit contains buffers, Cot DNA and streptavidin beads.

All xGen Blocking Oligos are compatible with any xGen Lockdown Panel, predesigned Gene Capture Pool or custom xGen Lockdown Probe set. Each oligo is individually synthesized and assessed by mass spectrometry for quality control.

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Image Variation SKU Price Quantity Add To Cart
Hybridization And Wash Kit, 16 Rxn 1080577 1,500.00
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Hybridization And Wash Kit, 96 Rxn 1080584 7,770.00
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Universal Blockers—TS Mix, 16 Rxn 1075474 4,560.00
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Universal Blockers—TS Mix, 96 Rxn 1075475 21,380.00
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Universal Blockers—TS Mix, 4 X 96 Rxn 1075476 104,500.00
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Universal Blockers—NXT Mix, 16rxn 1079584 4,379.66
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Universal Blockers—NXT Mix, 96rxn 1079585 21,306.55
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Universal Blockers—NXT Mix, 4x96rxn 1079586 78,124.08
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Description

The xGen Hybridization and Wash Kit has been optimized for in-solution target enrichments using xGen Lockdown Probes and Panels. The Cot DNA is used to block nonspecific cross hybridization, reducing background noise and improving signal specificity. Cot DNA is isolated from human placental DNA using methods that enrich for repetitive sequences such as the Alu and Kpn families, and sheared to a size range of 50–300 bp.

IDT currently offers three different ready-to-use, universal blocker reagents.

  • xGen Universal Blockers —TS Mix blocks Illumina LT (p5, p7 – 6 nt and 8 nt) and HT (i5, i7) adapters and is compatible with ligation-based library prep kits, such as Illumina TruSeq kits.
  • xGen Universal Blockers—10 bp TS Mix is compatible with ligation-based library prep kits that use 10 bp index barcodes.
  • xGen Universal Blockers— NXT Mix contains adapter blockers that are optimized for use with Illumina Nextera library prep kits.

Universal Blocking Oligos

  • Bind and block library dual- or single-index adapter sequences to reduce off-target capture during library enrichment
  • Reduce sequencing cost per sample by improving on-target performance
  • Worry-free formulations compatible with  Tecan Genomics DNA-Seq library prep kits
  • Reduce workflow steps with a convenient premixed ready-to-use predesignated mixes in liquid formulation

xGen Universal Blockers are offered in 16-, 96-, and 4 x 96-reaction formats for convenient experiment design and for use with xGen Lockdown Panels or xGen Lockdown Probe sets.

Additional information

Unit

Hybridization and Wash Kit, 16 rxn, Hybridization and Wash Kit, 96 rxn, Universal Blockers—TS Mix, 16 rxn, Universal Blockers—TS Mix, 96 rxn, Universal Blockers—TS Mix, 4 x 96 rxn, Universal Blockers—NXT Mix, 16rxn, Universal Blockers—NXT Mix, 96rxn, Universal Blockers—NXT Mix, 4x96rxn, Human Cot DNA, 150 µL, Human Cot DNA, 650 µL

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