PerkinElmer NEXTflex Amplicon Panels

PerkinElmer NEXTflex Amplicon Panels

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Amplicon panels are the technology of choice for small target panels, typically under 50kb of sequence. NEXTflex® Amplicon Panel Kits are available for sequence variation detection in numerous genes often associated with specific disorders. Pre-made panel kits are available for Inherited Disorders, Newborn and Infertility Syndromes, and Oncology Products.

They are the least expensive and most versatile option for sequencing, as fewer reads are required and different strategies can be employed to tackle the resolution of difficult genomic regions, such as those that are highly AT-rich, GC-rich, or possess paralogues or pseudogenes that may be masking the actual gene of interest.

Description

These panels include everything from primer pools, library prep reagents, clean-up beads, and barcodes that is necessary to construct libraries compatible with Illumina® and Ion Torrent™sequencing platforms.

Panels come in convenient reaction sizes that are easily scalable, easy to perform, and cost-effective, which makes them ideal for each lab’s particular needs. Both the workflow and NGS run conditions are equivalent for each panel, thereby allowing simple multiplexing of different panels on a single NGS run to maximize efficiency and minimize cost.

For pre-designed panels, click on the Amplicon Panel of interest below to learn more.     

PerkinElmer can also provide a custom panel up to ~300 targets and 30,000 kb of coverage. Service includes wet-bench testing and sequencing  to reach 100% coverage.    

For further information or to obtain a price quote, call us today at 02-58607138 ext. 2, or contact NGS@www.syntezza.com.  

NEXTflex Amplicon Panels for use with Genomic DNA Genes Covered
 Autism Spectrum Disorders PDE8B, EN2, NLGN4X, CDKL5, NLGN3, MECP2, RPL10
BRCA1/2 BRCA1, BRCA2
BRCA1/2 Plus-1 BRCA1, BRCA2, PALB2, CHEK2
CEBPA CEBPA
Colorectal Cancer -1 MLH1, MSH2
Colorectal Cancer-2 MSH6, PMS2
Congenital Adrenal Hyperplasia CYP21A2
Congenital Hyperinsulism ABCC8, GLUD1, KCNJ11, GCK, HADH, HNF4A, INS, INSR, PDX1, SLC16A1 & UCP2
CVD 22 hot spots for cardiovascular disease: MTHFR, F5, AGT, APOB, AGTR1, FGB, F13A1, LTA, SERPINE1, NOS3, JAK2, F2, ITGB3, APOE, PROCR, CBS
Cystic Fibrosis CFTR
Duchenne Muscular Dystrophy DMD
Epilepsy-2 SCN2A, SCN9A
Female Infertility FSHB, FSHR, LHB, LHCGR
HBOC-1 RAD51D, RAD51C, BRIP1
HBOC-2 PALB2, BARD1, TP53
Lysosomal Storage Diseases SUMF1, GLB1, IDUA, ARSB, GUSB, SMPD1, GALC, GALNS, GAA, GLA, IDS
Male Infertility AR, CATSPER1, CFTR, FSHR, LHCGR
Marfan Syndrome FBN1
Mediterranean Fever MEFV
MODY-1 HNF1A, GCK
MODY-2 HNF1B, HNF4A
MODY-3 (Diabetes-3) PDX1, NEUROD1, KLF11, CEL
MODY-4 (Diabetes-4) PAX4, INS, BLK
MODY-5 GCK, HNF1A, HNF1B, HNF4A
Myeloid Selected CDS from 21 genes
Nephrotic Syndrome-1 NPSH1, NPSH2, WT1
Nephrotic Syndrome-2 ARHGDIA, DGKE, LAMB2, PLCE1
Neurofibromatosis NF1, NF2
Neuronal Ceroid Lipofuscinoses CLN3, CLN5, CLN6, CLN8, CTSD, MFSD8, PPT1, TPP1
Obesity-1 LEP, LEPR, POMC, MC4R
Obesity-2 KSR2, SH2B1, SIM1
Periodic Fever-1 TNFRSF1A, NLRP3, MVK
Periodic Fever-2 ELANE, LPIN2, PSTPIP1
Phenylketonuria PAH
TP53 TP53
NEXTflex® Amplicon Panels – for Use with DNA Isolated from FFPE Samples
BRCA FFPE (covers BRCA1, BRCA2 genes)
TP53 FFPE (covers TP53 gene)