RNase A (Ribonuclease A) is a pancreatic endoribunuclease (13.7kDa monomer) that specifically cleaves single-stranded RNA at the 3′ end of pyrimidine residues.
RNase A (Ribonuclease A) is a pancreatic endoribunuclease (13.7kDa monomer) that specifically cleaves single-stranded RNA at the 3′ end of pyrimidine residues. The phosphodiester bond between the 5′-ribose of one nucleotide and the phosphate group of the 3′-ribose of an adjacent pyrimidine nucleotide is broken and the resulting 2′, 3′-cyclic phosphate is hydrolyzed to the corresponding 3′-nucleoside phosphate.
Plasmid DNA preparations, genomic DNA preparations, Ribonuclease protection assays, RNA removal from recombinant protein preparations, single-base mutation mapping in DNA or RNA by mismatch cleavage.
100 mg lyophilized powder purified from bovine pancreas.
Free of DNases and Proteases. No need to heat before use. Specific Activity > 100 Kunitz units per mg (>5000 Worthington units per mg of protein)
For short term, store the lyophilized powder and the dilution buffer at +4ºC separately. Before use, reconstitute RNase A at a desired concentration using a DNase-free buffer (e.g. TE), aliquot and store at -20ºC.
RNase A is active under a wide range of conditions. At low salt concentrations (<100 mM NaCl), RNA degrades single-stranded RNA, double-stranded RNA, as well as the RNA strand of RNA:DNA hybrids. At high salt concentrations (>300mM NaCl), RNA specifically cleaves single-stranded RNA. The recommended working solutions is 1-100 μg/ml (depending on the application). For the removal of RNA during preparation of plasmid DNA, a final concentration of 10 μg/ml is adequate.
RNase A can be inhibited by uridine 2′, 3′-cyclic vanadate, 5′-diphosphoadenosine 2′-phosphate, 5′-diphosphoadenosine 3′-phosphate, SDS, diethyl pyrocarbonate (DEPC), 4M guanidinium thiocyanate containing 0.1M β-mercaptoethanol, and heavy metals.
RNase A is inactivated by phenol/chloroform extraction.
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|GE011.0100||RNAse A (DNAse and Proteinase free, lyophilized,>5||100mg||₪100|