Adapters – TruSeq

Description

Unique Dual Indexed (UDI) Adapters 

• Illumina recommends using UDIs as a method to mitigate errors introduced by index-hopping. Suitable for both paired-end and single-end sequencing. Each UDI Adapter carries a unique 8-base barcode, 384 barcodes available.

• IDT xGen UDI Duplexes: Available as individual Adapters, delivered as a 15 uM solution (2nmole), sufficient for ~25 reactions.
• PerkinElmer NEXTflex UDI Kit: The kit includes 96 barcodes plus Primer Mix, supplied in 96-well plates, each well sufficient for 2 reactions, enabling 192 total reactions.

PerkinElmer Dual Index Adapter Kits – Best price for large projects 

• Kit contains 96 combinatorial dual indexed barcoded adapters plus Primer Mix
• Supplied in a 96-well plates, each well sufficient for 8 reactions, enabling 768 total reactions.
• Each indexed adapter carries an 8-base barcode on each end to enable unique identification.
• Two different kits available, pooling both enables up to 192 samples on a single flow cell

Single Index Adapters

• IDT TS-LT Single Index Adapter Kit A: Includes TS Amplification Primer Mix, plus the 12 indexed Adapters of Illumina Kit A.
• IDT TS-LT Single Index Adapter Kit B: Includes TS Amplification Primer Mix, plus the 12 indexed Adapters of Illumina Kit B.
  • Each indexed adapter is delivered annealed to the Universal Adapter, delivered at a concentration of 15 µM
  • Two sizes available: 600 pmol (for 8 rxns of 5µl) and 1.2 nmol (for 16 rxns of 5µl)
• PerkinElmer NEXTflex-HT Barcode Kit: The kit includes 96 unique single-index barcodes plus Primer Mix, supplied in a 96-well plates, each well sufficient for 2 reactions. Enables multiplexing up to 384 samples per flow cell lane.

IDT’s xGen Unique Molecular Indexed (UMI) Adapter Duplexes

• UMI Adapters are identical to the UDI Adapters but carry a short sequence of random bases, that incorporate a unique barcode onto each molecule within a given sample library. UMIs increase sensitivity of variant detection and are meant to reduce the rate of false-positive variant calls during SNP detection. UMI adapters are available at the same price as UDI adapters!