Xpert cDNA Synthesis Mastermix

Xpert cDNA Synthesis Mastermix

1,034.50

The Xpert cDNA Mastermix provides an efficient and fast method for the synthesis of high quality first strand cDNA from purified poly(A)+ mRNA or total RNA templates. The Xpert cDNA Mastermix (2X) is an optimized reaction mix containing a balanced concentration of oligo(dT) and random hexamer primers, dNTPs, and RNase inhibitor.

SKU: GK81.0100 Category:
GK81.0100

100 rxn

Description

Xpert Reverse Transcriptase (RNase H-) is a RNA-dependent DNA polymerase suitable for cDNA synthesis from long RNA templates. Xpert RTase has been optimized to perform under high temperatures (45ºC-55ºC), which facilitates the removal of secondary mRNA structures associated with high GC content. Together with the lack of RNase H activity, which ensures minimization of template degradation during long incubation times, Xpert RTase enables the preparation of long full-length cDNAs (up to 15kb).

The Xpert cDNA Mastermix provides an efficient and fast method for the synthesis of high quality first strand cDNA from purified poly(A)+ mRNA or total RNA templates. The Xpert cDNA Mastermix (2X) is an optimized reaction mix containing a balanced concentration of oligo(dT) and random hexamer primers, dNTPs, and RNase inhibitor. Note that, since the mastermix already contains primers, it cannot be used with gene-specific primers. First strand cDNA can be directly used as template in PCR.

 

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Product Info – Xpert cDNA Synthesis Mastermix

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Reference Description Quantity Price Add to Cart
GK81.0100 Xpert cDNA Synthesis Mastermix 100 rxn ₪100