Xpert Fast DNA polymerase (with dNTPs)

Description

Main application: routine PCR with extreme speed

Xpert Fast DNA polymerase is a robust enzyme, ideal for daily applications like genotyping and screening, amplifying with extreme speed, yield and consistency.
Xpert Fast DNA polymerase has 5’-3’ exonuclease activity, but no 3’-5’ exonuclease (proofreading) activity. PCR products generated with this enzyme are A-tailed, and can thus be cloned into TA cloning vectors.
The extreme speed of Xpert Fast DNA polymerase allows the use of an extension rate of 4-8 kb/min, making this the ideal choice for consistent results in fast routine PCR amplifications.

Performance highlights:
– routine amplifications with extreme speed (without performance loss)

Properties:
Amplicon size:  up to 5kb
Extension Rate: 4-8 kb/min
Hotstart:            No
A-overhang:      Yes

Applications:
– Fast routine PCR
– TA Cloning
– Genotyping

Components:
– Kit version with dNTPs included (in the buffer)
– Mastermix available with (red) dye

Storage:
-20ºC for at least 1 year. No loss of performance is detected after 20 freeze/thaw cycles.