Xpert Fast SYBR

Xpert Fast SYBR

143.003,364.50

For high performance, fast cycling, qPCR with SYBR Xpert Fast SYBR consists of the combination of a highly efficient enzyme with a novel low inhibitory technology, allowing for extremely high sensitivity and specificity.

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Image Variation SKU Price Quantity Add To Cart
xpert-fastsybr Xpert Fast SYBR (Uni) - 1ml (100 rxns) 143.00
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xpert-fastsybr Xpert Fast SYBR (Uni) - 5ml (500 rxns) 686.00
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xpert-fastsybr Xpert Fast SYBR (Uni) - 25mL (2500 rxns) GE20.2501 3,274.50
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xpert-fastsybr Xpert Fast SYBR (fluorescein) - 1ml (100 rxns) 143.00
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xpert-fastsybr Xpert Fast SYBR (fluorescein) - 5ml (500 rxns) GE20.0100 686.00
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xpert-fastsybr Xpert Fast SYBR (fluorescein) - 25mL (2500 rxns) GE212501 3,364.50
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Description

Main application: extremely high sensitivity and specificity qPCR with SYBR

Xpert Fast SYBR (uni) 2X mastermix contains all components, except primers and template, for the amplification and detection of DNA in qPCR. It consists of the combination of a highly efficient enzyme with a novel low inhibitory technology. The intercalating dye used in this mastermix causes little to no inhibition of the PCR reaction thus allowing for extremely high sensitivity and specificity, as well as preventing the formation of unwanted primer-dimers and non-specific products.

Xpert Fast SYBR (uni) can be used to quantify virtually any DNA target, including extremely low-copy number targets, with minimal effort and optimization.

Xpert Fast SYBR (uni) 2X mastermix is supplied with a separate vial of ROX reference dye, so it can be used with most real-time PCR instruments.

 

Properties:
– Excellent signal with low PCR inhibition
– Early Ct values – Rapid extenstion rate
– Extreme sensitivity – increased limit of detection
– Allows for standard and fast cycling
– Compatible with all qPCR machines (see FAQs for compatibility)

Applications:
– Absolute quantification
– Gene expression analysis
– High throughput qPCR
– Low-copy number target genes

ge20-melt-actg1
Amplification and melt trace of ACTG1 (Actin Gamma-1). Comparison between Xpert Fast SYBR (orange), and Market Leader (black).

Performance:

 

Components:
All Xpert Fast SYBR versions and sizes are supplied in multiples of 1mL, for your convenience!

– Xpert Fast SYBR (uni)
Xpert Fast SYBR (uni) is supplied as a convenient 2x mastermix containing all required components except specific primers. 1mL is enough for 100 reactions of 20μl.
Supplied also with a separate vial of 100X (50µM) ROX solution. Depending on the PCR equipment (see FAQs), prior to use, one should add 2µl of ROX (LowROX (final concentation of 50nM), 20µl (HighROX (final concentation of 500nM)), or nothing (No ROX). Once ROX has been added, the mastermix might be used immediately or stored at -20ºC.

– Xpert Fast SYBR (fluorescein)
Xpert Fast SYBR (fluorescein) is supplied as a convenient 2x mastermix containing all required components, including 20nM fluorescein, except specific primers. 1mL is enough for 100 reactions of 20μl.
This mastermix is for instruments equipped with a well-factor correction feature (see FAQs).

Storage:
-20ºC for at least 1 year.

Additional information

Item

Xpert Fast SYBR (Uni), Xpert Fast SYBR (fluorescein)

Unit

1ml (100 rxns), 5ml (500 rxns), 25mL (2500 rxns)

Documents

Datasheet – Xpert Fast SYBR (uni)

Datasheet – Xpert Fast SYBR (fluorescein)

Xpert flyer

Xpert qPCR selection table

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