Xpert HighFidelity DNA polymerase (with dNTPs)
For amplifications with extremely low error-rate Xpert HighFidelity DNA polymerase is a robust enzyme with enhanced DNA binding, resulting in improved processivity, yield, and extremely low error-rate (50x lower than Taq DNA polymerase)
Main application: high-fidelity amplification with enhanced speed and robustness
Xpert HighFidelity DNA polymerase is a robust enzyme with enhanced DNA binding, resulting in improved processivity, yield, and extremely low error-rate, ideal for applications such as high-fidelity PCR, site-directed mutagenesis, crude sample PCR, blunt-end cloning, among others, where robustness and proof-reading are important.
The Xpert HighFidelity DNA polymerase has an error-rate of approximately 1 error per 4.5×107 nucleotides incorporated, which is 50x lower than Taq DNA polymerase.
The velocity of Xpert HighFidelity DNA polymerase allows the use of an extension rate of up to 2kb/min, making this also an ideal choice for consistent results in fast PCR amplifications.
– extremely low error-rate (50X lower then Taq)
– robust amplification even for complex targets
Amplicon size: up to 10kb
Extension Rate: 2kb/min
Fidelity: 50x (compared to Taq)
– High-Fidelity PCR
– blunt-end cloning
– site-directed mutagenesis.
– Kit version with dNTPs included (in the buffer)
-20ºC for at least 1 year.
|Reference||Description||Quantity||Price||Add to Cart|
|GE07.0250||Xpert HighFidelity DNA polymerase (with dNTPs)||250 U||₪100|