Xpert HighFidelity DNA polymerase (with dNTPs)

Xpert HighFidelity DNA polymerase (with dNTPs)

630.00

For amplifications with extremely low error-rate Xpert HighFidelity DNA polymerase is a robust enzyme with enhanced DNA binding, resulting in improved processivity, yield, and extremely low error-rate (50x lower than Taq DNA polymerase)

SKU: GE07.0250 Category:
GE07.0250

250 U

Description

Main application: high-fidelity amplification with enhanced speed and robustness

Xpert HighFidelity DNA polymerase is a robust enzyme with enhanced DNA binding, resulting in improved processivity, yield, and extremely low error-rate, ideal for applications such as high-fidelity PCR, site-directed mutagenesis, crude sample PCR, blunt-end cloning, among others, where robustness and proof-reading are important.
The Xpert HighFidelity DNA polymerase has an error-rate of approximately 1 error per 4.5×107 nucleotides incorporated, which is 50x lower than Taq DNA polymerase.
The velocity of Xpert HighFidelity DNA polymerase allows the use of an extension rate of up to 2kb/min, making this also an ideal choice for consistent results in fast PCR amplifications.

Performance highlights:
– extremely low error-rate (50X lower then Taq)
– robust amplification even for complex targets

Properties:
Amplicon size:  up to 10kb
Extension Rate: 2kb/min
Hotstart:            No
A-overhang:       No
Fidelity:             50x (compared to Taq)

Applications:  
– High-Fidelity PCR
– blunt-end cloning
– site-directed mutagenesis.

Components:
– Kit version with dNTPs included (in the buffer)

Storage:
-20ºC for at least 1 year.

Documents

Datasheet – Xpert HighFidelity DNA Polymerase (with dNTPs)

Price Quote

Reference Description Quantity Price Add to Cart
GE07.0250 Xpert HighFidelity DNA polymerase (with dNTPs) 250 U ₪100