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Methyl-SEQ
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Select Starting Material
Sample Extraction
Extracted DNA
Sample requiring standard DNA extraction
FFPE sample requiring DNA extraction
Sample requiring cell free DNA extraction
Enter number of samples
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Select Methyl-Seq Type
Library Prep
Whole genome bisulfate, Standard input DNA (200ng – 1000ng)
Whole genome oxidated bisulfateisulfate, Standard input DNA (200ng – 1000ng)
Reduce representation bisulfate, Standard input DNA (200ng – 1000ng)
Reduce representation oxidated bisulfate, Standard input DNA (200ng – 1000ng)
Whole genome bisulfate, Low input DNA, cfDNA, FFPE (1ng – 200ng)
Whole genome oxidated bisulfateisulfate, Low input DNA, cfDNA, FFPE (1ng – 200ng)
Reduce representation bisulfate, Low input DNA, cfDNA, FFPE (1ng – 200ng)
Reduce representation oxidated bisulfate, Low input DNA, cfDNA, FFPE (1ng – 200ng)
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Calculate Number of Reads
NGS Sequencing
Methyl-Seq read size = 2 X 150
Enter Number of GB Units
M
Note: Each GB Unit adds 3.4M Reads. Reduced Representation protocols require a complete lane of 106 GB Units (350 reads).
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Bioinformatics
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