Small RNA species generally include the most common and well-studied microRNA (miRNA), small interfering RNA (siRNA), and piwi-interacting RNA (piRNA), as well as small nucleolar RNA (snoRNA) and small nuclear RNA (snRNA).
Small RNA is a type of lowly abundant, short in length (<200 nt), non-protein-coding RNA that lacks polyadenylation. Small RNA populations can vary significantly among different tissue types and species. Generally, small RNAs are formed by fragmentation of longer RNA sequences with the help of dedicated sets of enzymes and other proteins.
NGS sequencing of Small RNA’s requires unique library prep kits and protocols specifically designed for Small RNA samples. The TriLink CleanTag Small RNA Library Prep Kit is the preferred tool for preparing Small RNA libraries from cfDNA and Formalin-Fixed Paraffin-Embedded (FFPE) samples.
The process requires sample of 10pg to 100ng and is performed at the NGS Syntezza lab for ₪ 600 per sample.